艾克学院
什么是“甲基化”？
我们如何精确探索身体内的变化？
什么是DNA甲基化？
DNA甲基化是肿瘤发生的早期事件，是肿瘤筛查的有效手段之一。DNA甲基化是指基因组胞嘧啶-鸟嘌呤二核苷酸（CpG）上的5位碳原子，在DNA甲基转移酶家族（DNMTs，DNMT1、DNMT3a和DNMT3b）的介导下，共价结合一个游离的甲基形成5-甲基胞嘧啶的过程[1]。
一管血发现肿瘤踪迹
携带着遗传信息和表观遗传学改变的肿瘤细胞坏死或凋亡后，会向外周血中释放片段化的基因组DNA，即循环肿瘤 DNA (ctDNA)[2]。通过捕获这些微量 ctDNA，检测其特异性肿瘤甲基化位点改变，相较于传统检测技术，可更早、 更精准的检测肿瘤的发生和发展信息[3]。
参考文献：
[1] Rajabi H , Tagde A , Alam M , et al. DNA methylation by DNMT1 and DNMT3b methyltransferases is driven by the MUC1-C oncoprotein in human carcinoma cells[J]. Oncogene, 2016.
[2]Wan, J.C.M. et al. (2017) Liquid biopsies come of age: to-wards implementation of circulating tumour DNA. Nat.Rev. Cancer 17, 223–238
[3]Gao Y, et al. Whole-genome bisulfite sequencing analysis of circulating tumour DNA for the detection and molecular classification of cancer. Clin Transl Med. 2022 Aug;12(8):e1014.
基本
文件
流程
错误
SQL
调试
- 请求信息 : 2024-11-15 20:09:56 HTTP/1.1 GET : aikelun.dowv.cn/academy.html?page=12
- 运行时间 : 0.474953s [ 吞吐率：2.11req/s ] 内存消耗：4,923.31kb 文件加载：156
- 查询信息 : 44 queries 0 writes
- 缓存信息 : 36 reads,2 writes
- 配置加载 : 75
- [ BEHAVIOR ] Run Closure @app_init [ RunTime:0.000030s ]
- [ CACHE ] INIT File
- [ BEHAVIOR ] Run \addons\cms\Cms @app_init [ RunTime:0.015329s ]
- [ BEHAVIOR ] Run Closure @app_init [ RunTime:0.015378s ]
- [ LANG ] D:\wwwroot\aikelun.dowv.cn\thinkphp\lang\zh-cn.php
- [ BEHAVIOR ] Run app\common\behavior\Common @app_dispatch [ RunTime:0.002684s ]
- [ ROUTE ] array (
'type' => 'method',
'method' =>
array (
0 => '\\think\\addons\\Route',
1 => 'execute',
),
'var' =>
array (
'addon' => 'cms',
'controller' => 'channel',
'action' => 'index',
),
)
- [ HEADER ] array (
'content-type' => '',
'content-length' => '0',
'x-original-url' => '/academy.html?page=12',
'user-agent' => 'Mozilla/5.0 AppleWebKit/537.36 (KHTML, like Gecko; compatible; ClaudeBot/1.0; +claudebot@anthropic.com)',
'host' => 'aikelun.dowv.cn',
'accept-encoding' => 'gzip, br, zstd, deflate',
'accept' => '*/*',
)
- [ PARAM ] array (
'page' => '12',
'diyname' => 'academy',
)
- [ RUN ] think\addons\Route->execute[ D:\wwwroot\aikelun.dowv.cn\vendor\karsonzhang\fastadmin-addons\src\addons\Route.php ]
- [ LANG ] D:\wwwroot\aikelun.dowv.cn\public/../application/common\lang\zh-cn\addon.php
- [ BEHAVIOR ] Run app\common\behavior\Common @addon_begin [ RunTime:0.006383s ]
- [ LANG ] D:\wwwroot\aikelun.dowv.cn\addons\cms\lang\zh-cn.php
- [ DB ] INIT mysql
- [ VIEW ] D:\wwwroot\aikelun.dowv.cn\addons\cms\view\tpl\list_acad.html [ array (
0 => 'config',
1 => 'user',
2 => 'site',
3 => '__CHANNEL__',
4 => '__FILTERLIST__',
5 => '__ORDERLIST__',
6 => '__PAGELIST__',
) ]
- [ BEHAVIOR ] Run \addons\cms\Cms @view_filter [ RunTime:0.000152s ]
0.621423s
